Despite the benefits of FSC-A certification, there are also challenges and limitations. These include:
The relationship between Area and Height is linear. If the cell is twice as bright, both A and H double. Despite the benefits of FSC-A certification, there are
Here, "FSC-A" is calculated from the image mask. While less common, the same principle applies: area vs. height (or aspect ratio) weeds out doublets and clusters. However, imaging provides the ultimate confirmation – you can literally see if it’s a doublet. Despite the benefits of FSC-A certification
Keep event rate under 1,000-2,000 events/second. High speed distorts FSC-A due to pulse overlap. both A and H double. Here